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RIF proteins increase their association to deregulated telomeres, whereas RAP1 does not. ChIP assays were performed on asynchronous haploid cultures that had either TLC1 (−) or tlc1-476A/TLC1 (+) at the TLC1 locus, or contained a deletion at the RIF1 locus. Epitopes detected are indicated. Antibodies used for each strain are described under MATERIALS AND METHODS. The percentage of immunoprecipitated telomeric DNA as a function of total telomeric DNA input is shown.
