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. 2003 Feb;14(2):658–669. doi: 10.1091/mbc.E02-03-0142

Figure 4.

Figure 4

RACK1 regulates focal adhesion organization. Transiently transfected cells were plated onto coverslips coated with 20 μg/ml fibronectin. (A) Representative pictures that show the distribution of focal adhesions in cells that expressed pEGFP-N1 (a and b), pEGFP-N1-RACK1 (c and d), pEGFP-N1-RACK 1-203 (e and f), or pEGFP-N1-RACK Y246F (g and h). Vinculin staining is shown in the left column (a, c, e, and g) and GFP fluorescence is shown in the right column (b, d, f, and h). Bar, 20 μm. (B) Quantification of focal adhesion distribution. Blinded observers scored 50–100 GFP-positive cells from three separate experiments. Error bars represent the SD. *p < 0.01 in a Student's t test (in comparison with control).