Figure 6.
RACK1 localization to peripheral adhesion complexes requires C-terminal sequences but not the Src-binding site. Cells were plated onto 5 μg/ml fibronectin except for D. (A) Cells stained for endogenous RACK1. The enlarged area is magnified two times from the original. (B) Cells that expressed RACK1-GFP (green) stained for endogenous RACK1 (red). Bar, 10 μm. (C) Cells that expressed RACK1-GFP plated on fibronectin. (D) Cells that expressed RACK1-GFP plated on poly-l-lysine. (E) Cell that expressed RACK 1-203-GFP. (F) Cell that expressed RACK Y246-GFP. Images show representative results observed in at least three separate experiments. Bar, 20 μm.