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. 2003 Feb;14(2):658–669. doi: 10.1091/mbc.E02-03-0142

Figure 7.

Figure 7

RACK1 localizes to a subset of peripheral complexes that contain paxillin and α-actinin but not vinculin or zyxin. (A) Cells were plated onto coverslips coated with 5 μg/ml fibronectin. Images were taken with a 60× objective and enlarged areas were magnified two times in comparison with the original. a, Cell that expressed RACK1-GFP (green) stained for vinculin (red). b, Cell that expressed RACK1-GFP (green) stained for paxillin (red). c, Cell that expressed RACK1-GFP (green) and zyxin-RFP (red). d, Cell that expressed RACK-RFP (red) and α-actinin-GFP (green). Images show representative results observed in at least three separate experiments. Bar, 20 μm. (B) RACK1 and RACK 1-203 coimmunoprecipitate with paxillin, but not vinculin. Cells were transiently transfected with pEGFP-N1 (GFP), pEGFP-N1-RACK 1-203 (1-203), or pEGFP-N1-RACK1 (RACK1). Lysates were immunoprecipitated with either an anti-GFP antibody or a nonspecific rabbit IgG and blotted for vinculin, paxillin, or GFP. Total cell lysate was stained for vinculin and paxillin. Blots show a representative result from three separate experiments.