Figure 5.

Chs2p dynamics in myo1Δ (healthy) cells. (A and C) Chs2p-GFP, GFP-Tub1p expressing myo1Δ (healthy) cells (RLY1674) were observed using three-dimensional (3D) confocal video microscopy, as described in MATERIALS AND METHODS. Two representative time-lapse series are shown, consisting of deconvolved two-dimensional projections of each 3D image. Note that cell outlines have been drawn in for clarity. White arrows indicate bud neck localized Chs2p-GFP. Scale bar, 3 μm. (B and D) Intensity profiles of bud neck localized Chs2p-GFP. A line was drawn through the Chs2p ring of the cells shown in A and C, respectively, at each of the indicated time points, and pixel intensity values along the line were obtained, as described in MATERIALS AND METHODS. The measurements were then plotted against distance along the line.