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Expression of NcPex20p restores growth of pex18Δpex21Δ cells on oleic acid. Tenfold dilutions of the wild-type, the untransformed pex18Δpex21Δ and the pex18Δpex21Δ strain expressing either ScPex18p or NcPex20p were spotted onto an oleic acid plate and incubated for 5 d at 30°C. Halos surrounding the spots indicate utilization of the fatty acid from the medium.
