Figure 4.

Function of NcPex20p resembles that of Pex18p. (A) Dependence of the NcPex20p interactions on ScPex7p. The indicated peroxins were tested in a two-hybrid assay for interaction with NcPex20p in the HF7c wild-type strain (left) and the otherwise isogenic pex7Δ strain (right). Interactions are indicated by the histidine prototrophy of two independent transformants at each case. (B) Accumulation of a Pex7p–Fox3p complex through substitution of Pex18p/Pex21p with NcPex20p in the docking mutant pex14Δ. The indicated strains were analyzed for the presence of Fox3p in the immunoprecipitate (20% of total) of myc-Pex7p by immunoblotting (top). The bottom panel shows 0.5% of the used total cell lysates.