Figure 6.

NcPex20p converts NcPex7p into a functional PTS2 receptor in S. cerevisiae. (A) Growth of a pex7Δ strain on oleic acid resumes in the concomitant presence of NcPex7p and NcPex20p. Tenfold dilutions of the wild-type, the untransformed pex7Δ as well as the pex7Δ strain transformed with either NcPEX7, NcPEX20, or NcPEX7 plus NcPEX20 were spotted onto oleic acid plates and incubated for 5 d at 30°C. Halo formation was observed in the strain coexpressing both N. crassa peroxins. (B) Expression of NcPex7p does not change in the presence of NcPex20p. Whole-cell extracts of the indicated strains that had been induced by oleic acid were analyzed for the expression level of NcPex7p using Pex7p-specific antibodies. The amount of NcPex7p exceeded that of native ScPex7p (lane 1) but was comparable in the absence or presence of NcPex20p (lanes 3 and 4).