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. 2003 Mar;15(3):626–638. doi: 10.1105/tpc.007922

Figure 1.

Figure 1.

Molecular Characterization of the ada2b-1 Mutant.

(A) Scheme of the T-DNA insertion in the Arabidopsis ADA2b gene, indicating the positions of exons (black boxes), introns (white boxes), HindIII sites (H), PCR primers (st628 and st637), and the T-DNA insertion.

(B) DNA gel blot analysis of genomic DNA from wild-type (+/+), heterozygous (+/−), and homozygous ada2b-1 (−/−) plants digested with HindIII and probed with radiolabeled fragments of the ADA2b gene or the neomycin phosphotransferase gene (NPTII) from the T-DNA.

(C) RNA gel blot analysis of total cellular RNA from wild-type (+/+) and homozygous ada2b-1 (−/−) plants probed with radiolabeled fragments of the 5′ half of the ADA2b cDNA.

(D) RT-PCR analysis of total cellular RNA from wild-type (+/+), heterozygous (+/−), and homozygous ada2b-1 (−/−) plants using primers that flank the T-DNA insertion site.