Figure 5.

Transcript Levels of COR and CBF Genes in ada2b-1 and gcn5-1 Mutants.

(A) RNA gel blot analysis of total RNA prepared from whole seedlings of control Arabidopsis Wassilewskija-2 wild-type (WT) and ada2b-1 and gcn5-1 mutant plants. Plants were grown for 14 days at 20°C and then cold treated at 5°C for the times indicated. The RNA gel blots were hybridized with a probe corresponding to CBF1 and then stripped and hybridized with probes corresponding to COR47, COR6.6, COR78, or COR15a. Hybridization with a probe representing EF-1α, a constitutively expressed gene, was used to normalize signals from the COR and CBF genes.

(B) Quantitative representation of the relative expression of COR genes (COR/EF-1α expression) during a low-temperature time course. wt, wild type.