Table 1.
Oligonucleotides Used as PCR Primers for the Identification and Characterization of T-DNA Disruption Mutants of ADA2b and GCN5 Genes
| Primer Name | Sequence | Location | Stranda |
|---|---|---|---|
| JL202 | 5′-CATTTTATAATAACGCTGCGGACATCTAC-3′ | T-DNA left border | NA |
| XR-2 | 5′-TGGGAAAACCTGGCGTTACCCAACTTAAT-3′ | T-DNA right border | NA |
| ST461 | 5′-AGGGTTCTTCTTCTCTGTGGTTTCGCATA-3′ | 5′ end of ADA2b | NT |
| ST468 | 5′-GGTTTAGATTGTGTGGCTCTGATTCACCT-3′ | 3′ end of ADA2b | T |
| ST628 | 5′-ACTCCTCACAAATGTGATCACCCATACCG-3′ | Exon 3 of ADA2b | NT |
| ST637 | 5′-CTCCATCTCCGCCAAGAGTTGCTCAG-3′ | Exon 9 of ADA2b | T |
| ST496 | 5′-GAATATTCCGTTCGTCGGCGTAGTCTTTC-3′ | 5′ end of GCN5 | NT |
| ST497 | 5′-AAATGACCCATGACGCTCATTCTCTTCAC-3′ | 3′ end of GCN5 | T |
| ST646 | 5′-AGTGGGGGCACACTCGTTTCAAATTATTC-3′ | Exon 10 of GCN5 | NT |
| ST647 | 5′-TCCGCAACAAACATATCCAATGTCACGTA-3′ | Exon 12 of GCN5 | T |
| ST466 | 5′-TTGAGATTTAGCACCAGATTGGAGACCTG-3′ | Exon 13 of GCN5 | T |
a
Primers used to prime DNA synthesis on the template (T) or nontemplate (NT) strand. NA, not applicable.