Figure 2.

Overexpression of 70z-Cbl promotes the loss of cell–cell contacts. (A) Parental MDCK cells, unstimulated or stimulated with 5 U/ml HGF for 24 h, as well as stable cell lines expressing c-Cbl (clone 8) and 70z-Cbl (clone 20), were grown on glass coverslips in DMEM containing 10% FBS. Cells were treated for 10 min with CSK buffer, fixed in 3.7% formaldehyde, and then labeled with anti-E-cadherin antibody followed by Cy3-conjugated anti-mouse antiserum (a, c, e, and g). Matching phase-contrast images are shown (b, d, f, and h). Photographs were taken at a magnification of ×63. (B) Whole cell lysates were separated on an 8% SDS-PAGE gel and immunoblotted with anti-E-cadherin antibody.