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. 2003 Mar;15(3):681–693. doi: 10.1105/tpc.007831

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Copyright © 2003, American Society of Plant Biologists

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Figure 6. — Histochemical Localization of GUS Activity in 7- and 14-Day-Old Wild-Type and emf1 Seedlings Harboring the LFY::GUS or AP1:: GUS Transgene.

GUS activity is indicated by blue color. Bars = 0.5 mm.

(A) LFY::GUS wild type (WT) at 7 DAG, showing GUS activity at the shoot tip (arrow).

(B) LFY::GUS wild type at 14 DAG, showing GUS activity at the meristematic region of the shoot tip (arrow).

(C) LFY::GUS emf1-1 at 7 DAG, showing GUS activity at the shoot tip (arrow).

(D) LFY::GUS emf1-1 at 14 DAG, showing GUS activity at the shoot tip.

(E) LFY::GUS emf1-2 at 7 DAG, showing GUS activity at the shoot tip (arrow).

(F) LFY::GUS emf1-2 at 14 DAG, showing GUS activity in a patch of the carpelloid tissue (arrow).

(G) AP1::GUS emf1-2 at 7 DAG, showing GUS activity in the shoot tip, cotyledons, and hypocotyl.

(H) AP1::GUS emf1-2 at 14 DAG, showing GUS activity in the carpelloid structure and the papillae tissue developed at the base of the cotyledons (arrows).