Figure 7.

CSN5 and CSN6 Contact Each Other Physically within CSN.

(A) The formaldehyde treatment of cauliflower inflorescences generates a high molecular mass protein complex (top arrow at right) on SDS-PAGE that is recognized by the antibodies to AtCSN5 and AtCSN6 (indicated at bottom).

(B) The high molecular mass complex (top arrow at right) can be immunoprecipitated by antibodies to both AtCSN5 and AtCSN6 but not by antibodies to AtCSN7. Antibodies used for immunoprecipitation (IP) are indicated at top. The immunoblot analysis was performed with the antibodies indicated at bottom.