Figure 10.

Altered Tolerance of OsMAPK5-OX and OsMAPK5-RI Transgenic Plants to Cold, Salt, and Drought.

(A) Percentage of surviving seedlings after treatment with cold (4°C for 3 days followed by normal growth conditions for recovery), salt (200 mM NaCl for up to 4 days), or drought (withholding water for up to 6 days). At least 40 hygromycin-positive T1 transgenic seedlings were used in each experiment. All experiments were repeated twice. Statistical analysis (t test) was performed to evaluate the levels of cold, salt, and drought tolerance based on the percentage of surviving seedlings in the overexpression or suppression lines versus the control line after the abiotic treatments. See supplemental data online for photographs of rice seedlings after cold, salt, and drought treatments.

(B) MBP in gel kinase assay of immunoprecipitated OsMAPK5 from mixed leaf tissue sampling at different times under cold (6, 12, and 24 h), salinity (6, 12, and 24 h), or drought (2, 3, and 4 day) stresses. The relative MBP kinase activities of control and transgenic lines and the control line were calculated based on phosphorimager quantification of band intensity.