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. 2003 Mar;15(3):745–759. doi: 10.1105/tpc.008714

Figure 4.

Figure 4.

Induction of OsMAPK5, Its Protein, and Kinase Activity by Abscisic Acid and Wounding.

Experiments were repeated twice using samples from independent treatments.

(A) RNA gel blot analysis of OsMAPK5 expression in 2-week-old seedlings treated with 0.1 mM abscisic acid (ABA), 1 mM SA, 0.1 mM JA, or wounding. Total RNAs were extracted at the times specified. The same blots were probed with the cDNA of PBZ1, a rice defense gene that encodes an intracellular PR10 protein (Midoh and Iwate, 1996), to assess the effectiveness of the chemical treatments because PBZ1 is induced by abscisic acid, SA, and JA (Lee et al., 2001).

(B) Immunoblot analysis of OsMAPK5 in 2-week-old seedlings treated with 0.1 mM abscisic acid, 1 mM SA, 0.1 mM JA, or wounding.

(C) MBP in gel kinase activity of immunoprecipitated OsMAPK5 from 2-week-old seedlings treated with 0.1 mM abscisic acid, 1 mM SA, 0.1 mM JA, or wounding.