Figure 8.
OsMAPK5-RI Lines Exhibited Enhanced Resistance to the Bacterial Pathogen B. glumae.
Leaf sheaths of 1-month-old control and T1 transgenic seedlings were inoculated with B. glumae (1 × 106 colony-forming units). At least 10 hygromycin-positive transgenic seedlings per line were used in each experiment. Experiments were repeated twice with similar results.
(A) Disease resistance evaluation based on lesion size at 7 days after inoculation. See supplemental data online for photographs of disease symptoms.
(B) Disease resistance evaluation based on bacterial growth in planta at 7 days after inoculation.
(C) MBP in gel kinase assay of immunoprecipitated OsMAPK5 from leaf tissues at 7 days after inoculation.