Figure 6.

SA-Mediated Suppression of MeJA-Induced PDF1.2 Expression through a Function of NPR1 in the Cytosol.

RNA gel blot analysis of the SA-responsive PR-1 gene and the MeJA-inducible PDF1.2 gene in wild-type Col-0, mutant npr1, and the NPR1-overexpressing transformants 35S:NPR1 (in npr1) and 35S:NPR1-HBD (in npr1). Seedlings were grown for 12 days on Murashige and Skoog (1962) medium with or without DEX (5 μM). Subsequently, the seedlings were transferred to fresh medium with (+) or without (−) 0.5 mM SA and 0.02 mM MeJA. Two days after induction, the seedlings were harvested for RNA gel blot analysis. Equal loading of RNA samples was checked by staining rRNA bands with ethidium bromide.