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. 2000 Oct;11(10):3411–3424. doi: 10.1091/mbc.11.10.3411

Figure 3.

Figure 3

mcs1 delays the initiation of mitosis. (A) mcs1-77 is synthetically lethal with cdc25-22. Wild-type, mcs1-77, cdc25-22, and mcs1-77 cdc25-22 cells were grown on YEPD at 28°C and then streaked onto YEPD plates at 28, 31, or 36°C, and the growth of the strains was monitored after 3 d at these temperatures. (B) mcs1-77 cdc25-22 arrested cells are highly elongated. mcs1-77 cells were crossed to cdc25-22 cells, and the resulting asci were subjected to tetrad dissection on YEPD plates at 31°C. Colonies from a tetratype that were genotypically mcs1-77 (left), cdc25-22 (middle), or mcs1-77 cdc25-22 (right) were visualized after 2 d of growth at 31°C.