Figure 3.

Effect of ATP and ATP-γ-S on composition of proteasome preparations. The entire purification of 19S caps from the RPT1^FH strain (RJD 1171) was carried out as described in the legend to Figure 1A in the absence of ATP (lane 1) or in the presence of either ATP (lane 2), or ATP-γ-S (lane 3). Similarly, purification of 26S proteasomes from the PRE1^FH strain (RJD 1144) was performed in the presence of ATP (lane 5) or in the presence of ATP-γ-S (lane 6). As a mock control, purification was carried out with extracts prepared from untagged RJD 487 cells (lane 4).