Figure 7.

Paf1 and Leo1 coimmunoprecipitate with 19S regulatory particles. Extracts were prepared from yeast strains that expressed Daq1^myc9 (RJD 1487), Paf1^myc9 (RJD 1486), and Leo1^myc9 (RJD 1484) or from strains that coexpressed these tagged proteins along with Rpt1^FH (RJD 1494, RJD1496, and RJD 1497, respectively). Extracts were immunoprecipitated with anti-Flag resin in the absence of ATP and immunoprecipitates were analyzed by immunoblotting with anti-myc monoclonal antibody. L refers to extract loaded for immunoprecipitation (3% of input), and B refers to the washed and SDS-eluted immunoprecipitates (Beads, 25% of bound).