FIG. 4.

Time course PCR-DGGE analysis of the nonsulfidogenic enrichment consortium MI-A exposed to 0.85 mM Cr(VI). 16S rDNA fragments were analyzed on a denaturing gel (35 to 70% denaturant; Sybr Gold stained). Each lane represents the community MI-A DNA at a specific time point in the absence and presence of 0.85 mM Cr(VI); 300 ng was loaded onto each lane. The PCR product used for preparing the inoculum was run on a different DGGE gel and presented alongside the time course experiment. It is apparent that bands in the inoculum correlate reasonably well with the time course experimental profile.