FIG. 2.
Experimental sensitivity and accuracy of the CFE and QPCR analysis method for quantifying Candida cells. Known numbers of fluorescently labeled C. albicans cells were collected by filtration on polyethylene terephthalate filters and corroborated by solid-phase cytometry and microscopy. The counted cells on the filters were extracted in the presence of G. candidum reference cells by using the CFE method, and C. albicans cells were quantified by QPCR and ΔΔCT comparative CT analyses. The regression of log cell numbers per filter determined by direct counts on log cell numbers determined by QPCR analysis is represented by a single solid line. The dashed lines represent the 95% confidence limits for the individual results.