TABLE 3.

Assay, amplification efficiency, and mean extrapolated cell detection limits of Candida species tested by using the QPCR method^a

Yeast species	Assay	Amplification efficiencyb	Mean cell detection limit ± SDb
C. albicans	Calb	1.95	1.07 ± 0.74
C. glabrata	Cglab	2.00	0.47 ± 0.11
C. krusei	Ckru	1.89	0.55 ± 0.091
C. lusitaniae	Clus	1.90	0.73 ± 0.46
C. parapsilosis	Cpar	1.92	0.25 ± 0.02
C. tropicalis	Ctrop	1.91	0.083 ± 0.04

^aCell detection for all other species tested, as listed in Table 1, were negative. DNA extracts from at least 10⁴ cells of each of the nontarget species were analyzed.

^bAmplification efficiencies and extrapolated cell detection limits were determined as described in the text and illustrated in Fig. 1.