TABLE 3.
Primers used in this study
| Target gene and primer direction | Sequence (5′→3′) | GenBank accession no. | Location (nucleotide) | Amplicon length (bp) | Annealing temp (°C) |
|---|---|---|---|---|---|
| ail | |||||
| Forward | TAATGTGTACGCTGCGAG | M29945 | 00544-00894 | 351 | 57 |
| Reverse | GACGTCTTACTTGCACTG | ||||
| ystA | |||||
| Forward | ATCGACACCAATAACCGCTGAG | X65999 | 00093-00171 | 79 | 61 |
| Reverse | CCAATCACTACTGACTTCGGCT | U09235 | 01181-01259 | 79 | |
| ystB | |||||
| Forward | GTACATTAGGCCAAGAGACG | D88145 | 00143-00288 | 146 | 61 |
| Reversea | GCAACATACCTCACAACACC | ||||
| inv | |||||
| Forward | CGGTACGGCTCAAGTTAATCTG | M17448 | 00923-01105 | 183 | 61 |
| Reverse | CCGTTCTCCAATGTACGTATCC | ||||
| yadA | X13882 | 01024-01872 | 849 | ||
| Forward | CTTCAGATACTGGTGTCGCTGT | AF102990 | 00465-01313 | 849 | |
| Reverse | ATGCCTGACTAGAGCGATATCC | AF056092 | 56159-57007 | 849 | 60 |
| X13881b | 00749-01507 | 759 | |||
| X13883c | 00868-01548 | 681 | |||
| virF/lcrF | |||||
| Forward | GGCAGAACAGCAGTCAGACATA | AF102990 | 32162-32722 | 561 | 63 |
| Reverse | GGTGAGCATAGAGAATACGTCG | M86690d | 00874-00314 | 561 |
a
From Ramamurthy et al. (27).
b
Y. enterocolitica serotype O:8.
c
Y. pseudotuberculosis.
d
Yersinia pestis lcrF gene.