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Dot blot for cpe gene by use of DIG-labeled cpe-specific gene probe. Fifty or 25 μl of 18-h cell cultures of each C. perfringens isolate was added to separate grids. B1 and B2, positive control (strain NCTC 8239); D1 and D2, positive control (strain FD 1041); C1 and C2, negative control (strain FD1); E1 and E2, negative control (strain NCTC 3624). Columns 3 to 12, C. perfringens food isolates (50 μl).
