TABLE 3.
Media formulae supporting ≥10% sporulation of retail isolates of C. perfringens
| Medium and adjunct | No. of isolates with ≥10% sporulationa |
|---|---|
| DS-S | 19 (11-47)b |
| Caffeine | 7 (10-42) |
| Theobromine | 5 (10-37) |
| Papaverine | 7 (11-26) |
| Bile salts | 5 (12-33) |
| Sodium taurocholate | 2 (12-31) |
| Rumen fluid | 7 (11-48) |
| Sporulation factor | 5 (11-26) |
| DS-R | 25 (11-55) |
| Caffeine | 6 (11-53) |
| Theobromine | 5 (11-55) |
| Papaverine | 3 (11-25) |
| Bile salts | 4 (11-40) |
| Sodium taurocholate | 2 (11-36) |
| Rumen fluid | 3 (12-44) |
| Sporulation factor | 2 (10-25) |
a
Values corresponding to DS-S and DS-R are the number of isolates with ≥10% sporulation. Values corresponding to the adjuncts are the number of additional isolates for which each adjunct increased sporulation from <10% to ≥10%. The cultures of each isolate with the highest percentage of sporulation were kept for CPE analysis.
b
Values in parentheses indicate the range of the percentage of sporulation.