Genomic region around unc-13, with
predicted unc-13 transcripts and protein products. (A)
Genomic sequence indicating the locations of the major
unc-13 transcript and the extents of cosmids C44E1 and
ZK524. (B) Genomic sequence with introns and exons indicating the
production of three predicted unc-13 transcripts. Region
L (left) corresponds to the less conserved 5′ end of
unc-13, and region R (right) corresponds
to the more highly conserved 3′ end of unc-13. Region
M (middle) corresponds to an exon that was not
originally identified in the published sequence of
unc-13. Asterisks mark positions of alternative
splicing. (C) Proteins expressed from the predicted transcripts are
shown. cDNA library screens and RACE products indicate the existence of
unc-13 products. The cDNAs yk50c3 (GenBank accession
number D67349), yk16b7 (GenBank accession numbers D26913 and D35032),
and yk8h12 (GenBank accession number D37721) were isolated by Yuji
Kohara from a dT-primed C. elegans library. The
remaining cDNAs (RM#418p, RM#384p, RM#419p, RM#385p, and RM#386p) were
isolated in our laboratory from a random primed C.
elegans library. 5′ RACE and cRACE (Maruyama et
al., 1995) were used to produce RACE products.