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. 2000 Oct;11(10):3441–3452. doi: 10.1091/mbc.11.10.3441

Figure 1.

Figure 1

Genomic region around unc-13, with predicted unc-13 transcripts and protein products. (A) Genomic sequence indicating the locations of the major unc-13 transcript and the extents of cosmids C44E1 and ZK524. (B) Genomic sequence with introns and exons indicating the production of three predicted unc-13 transcripts. Region L (left) corresponds to the less conserved 5′ end of unc-13, and region R (right) corresponds to the more highly conserved 3′ end of unc-13. Region M (middle) corresponds to an exon that was not originally identified in the published sequence of unc-13. Asterisks mark positions of alternative splicing. (C) Proteins expressed from the predicted transcripts are shown. cDNA library screens and RACE products indicate the existence of unc-13 products. The cDNAs yk50c3 (GenBank accession number D67349), yk16b7 (GenBank accession numbers D26913 and D35032), and yk8h12 (GenBank accession number D37721) were isolated by Yuji Kohara from a dT-primed C. elegans library. The remaining cDNAs (RM#418p, RM#384p, RM#419p, RM#385p, and RM#386p) were isolated in our laboratory from a random primed C. elegans library. 5′ RACE and cRACE (Maruyama et al., 1995) were used to produce RACE products.