Anti-UNC-13L antibody stains a form of
UNC-13 protein that is synaptic but is unlikely to be on synaptic
vesicles. C. elegans were double stained with
anti-UNC-13L (left) and anti-UNC-17/VAChT (right). (A) Anti-UNC-13L in
an adult wild-type head stains the major synaptic regions, including
the nerve ring (NR), ventral nerve cord (VNC), dorsal nerve cord (DNC),
and sublateral nerve cords (SNC). (B) Anti-UNC-17/VAChT stains a subset
of the region stained by anti-UNC-13L. (C and D) A slightly higher
magnification shows punctate staining with both antibodies in the
dorsal nerve cord and sublateral nerve cords in the body. (E and F) A
higher magnification shows the colocalization of anti-UNC-13L (E) and
anti-UNC-17/VAChT (F) in synaptic regions (arrowheads) in one of the
sublateral nerve cords in the head. (G and H) In an adult
unc-104 mutant, staining of anti-UNC-13L is somewhat
less intense but remains synaptic, whereas anti-UNC-17/VAChT is now
localized in neuronal cell bodies (CB). Anterior is left; bars, 10 μ
m.