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. 2003 Mar;69(3):1817–1826. doi: 10.1128/AEM.69.3.1817-1826.2003

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FIG. 1. — Analysis of acyl-HSLs produced by P. chlororaphis SPR044 and TnMod insertion derivatives. Ethyl acetate extracts from cell supernatants and synthetic reference acyl-HSLs (lane C₄, BHSL; lane C₆, HHSL; lane C₈, OHSL) were separated on C₁₈ reversed-phase TLC plates in methanol-water (60:40). Bioassays were conducted with the reporter strains A. tumefaciens A136(pCF218)(pCF372) (A) and phzI-negative P. aureofaciens 30-84I (B). Lane 1, SPR144; lane 2, SPR244; lane 3, SPR344; lane 4, SPR544; lane 5, SPR644; lane 6, SPR744; lane 7, SPR844; lane 8, SPR044. The arrowheads indicate signals induced by acyl-HSLs from strain SPR044. The arrows indicate the direction of solvent migration.