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Stable Wp85α transfection of control Rat-1 fibroblasts accelerates fluid-phase endocytosis. Control (○) or Wp85α fibroblasts (▴) were preincubated for 30 min in DMEM without serum, further incubated for the indicated times of pulse in the same medium containing HRP (A) or for 30 min with HRP, washed, and then chased for the indicated times in DMEM (B). Intracellular tracer accumulation is calculated as in Figure 1. Values are means ± SD of four dishes.
