TABLE 1.
Compositions of major fatty acids of L8-14C yeast cells transformed with plasmids encoding integral membrane desaturasesa
| Transformant | Fatty acid content (%)b
|
|||
|---|---|---|---|---|
| Saturated
|
Monounsaturated
|
|||
| C16:0 | C18:0 | C16:1 | C18:1 | |
| Δ9Z | ||||
| OLE1 | 45.5 ± 2.2 | 4.7 ± 2.4 | 34.9 ± 0.8 | 14.9 ± 1.0 |
| HzeaKPSE | 49.5 ± 5.5 | 7.9 ± 2.2 | 31.7 ± 5.6 | 11.0 ± 2.0 |
| TniNPVE | 46.9 ± 4.0 | 8.6 ± 3.9 | 12.8 ± 1.9 | 31.7 ± 5.8 |
| Δ11Z | ||||
| HzeaLPAQ | 45.6 ± 3.6 | 11.9 ± 2.8 | 42.6 ± 6.3 | |
| TniLPAQ | 49.7 ± 4.8 | 12.5 ± 0.1 | 41.8 ± 11.8 | 11.2 ± 1.5 |
a
Analyzed at mid-log phase (2 × 107 to 3 × 107 cells per ml) in two separate experiments.
b
Determined from the peak areas of methyl esters.