Figure 1.

SNAP23 is targeted to vimentin filaments in fibroblasts. (A) Confocal sections of human and murine fibroblasts in primary culture double-stained for vimentin and SNAP23 by using two different anti-SNAP23 antibodies: TG7, directed against the full recombinant human protein, and α-Nter, directed against the first 17 N-terminal amino acids of human SNAP23. In both cases, SNAP23 immunoreactivity appears as discontinuous spots along vimentin filaments. (B) Confocal section of HeLa Cells double-stained for SNAP23 (with TG7) and vimentin. SNAP23 immunoreactivity is present both at the plasma membrane and along vimentin filaments.