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. 2000 Oct;11(10):3485–3494. doi: 10.1091/mbc.11.10.3485

Figure 5.

Figure 5

Figure 5

(A) Western blot analysis of extracts of fibroblasts from wild-type (V+) and vimentin knockout (V−) mice. Vimentin is expressed only by V+ fibroblasts, whereas SNAP23 and syntaxin 4 (Stx4) equally expressed by V+ and V− cells. Note the high specificity of the anti-SNAP23 (TG7) antibody. (B) SNAP23 localizes at the plasma membrane in V− fibroblasts. V+ and V− fibroblasts were double-stained for SNAP23 and vimentin. SNAP23 colocalizes with vimentin in V+ cells but is found in punctate structures mostly at the plasma membrane in V− cells.