Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Vimentin-associated SNAP23 is Triton X-100 insoluble. V+ and V− fibroblasts were permeabilized with Triton X-100 (Triton X-100 prepermeabilization) or not (no prepermabilization) before methanol fixation, double-stained for SNAP23 and vimentin, and observed on the confocal microscope by using the same parameters in all cases. The images were then treated equally. SNAP23 immunoreactivity is extracted by Triton X-100 prepermeabilization in V− cells but not in V+ cells.
