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. 2006 Jun 16;7(7):716–721. doi: 10.1038/sj.embor.7400730

Figure 3.

Figure 3

ORC interacts with TRF2. (A) Coomassie stain of purified GST, GST–hRap1, GST–TRF2 and GST–TRF1. (B) Western blot with ORC2 antibodies of GST pull-down assays using Raji nuclear extract and various GST proteins shown in (A). Ethidium bromide (EtBr; 100 μg/ml) was included in the binding reactions, as indicated. (C) Co-immunoprecipitation with antibodies to EBNA1, TRF2 or control IgG. Immunoprecipitates were assayed by western blot with antibodies to EBNA1, TRF2, ORC2 or control PCNA. (D) Co-immunoprecipitation assay with anti-TRF2 and western blot with anti-ORC2 in Raji (top) or HeLa (bottom) nuclear extracts. (E) FLAG–ORC2 was purified from stable cell lines and assayed for direct interaction with purified GST or GST–TRF2 by western blot with anti-FLAG. EBNA1, Epstein–Barr virus nuclear antigen 1; GST, glutathione S-transferase; IB, immunoblotting; IP, immunoprecipitation; ORC, origin recognition complex; PCNA, proliferating-cell nuclear antigen; TRF, telomere repeat factor.