Neighbor-joining tree based on deduced amino acid sequences of chlorocatechol 1,2-dioxygenase genes derived from the clone libraries obtained in this study (boldface type) and amino acid sequences from the NCBI database. The scale bar represents an estimated change of 5%. The clone designations indicate the sampling stations. For example, clone RB3C3 was obtained from sampling port 3 of reactor 1b. C1, C2, C3, etc. indicate the consecutive numbers of the clones.