TABLE 1.
Detection by RT-PCR of chlorocatechol dioxygenase genes from groundwater and aquifer samples retrieved from different sectors of in situ reactor columns 1a (RA4) and 1b (Z, RB3, RB4, RB5, RB7, RB9)
| Sample type | Operation day | Gene | RT-PCR products detected at the following sampling stationsa:
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Z | RB3 | RB4 | RB5 | RB7 | RB9 | RA4 | |||
| Ground water | 733 | Chlorocatechol 1,2-dioxygenase | + | + | + | NDb | + | ND | ND |
| Chlorocatechol 2,3-dioxygenase | + | − | − | ND | − | ND | ND | ||
| 775 | Chlorocatechol 1,2-dioxygenase | + | + | + | − | − | ND | ND | |
| Chlorocatechol 2,3-dioxygenase | +c | − | − | − | − | ND | ND | ||
| Aquifer | 803 | Chlorocatechol 1,2-dioxygenase | +c | ND | − | − | − | − | |
| Chlorocatechol 2,3-dioxygenase | +c | ND | − | − | − | − | |||
| 845 | Chlorocatechol 1,2-dioxygenase | +c | ND | ND | + | + | ND | ||
| Chlorocatechol 2,3-dioxygenase | + | ND | ND | − | − | ND | |||
| 859 | Chlorocatechol 1,2-dioxygenase | + | + | + | + | + | ND | ||
| Chlorocatechol 2,3-dioxygenase | + | +c | − | − | − | ND | |||
| 895 | Chlorocatechol 1,2-dioxygenase | + | + | + | + | − | − | ||
| Chlorocatechol 2,3-dioxygenase | + | − | − | − | − | − | |||
a
The sampling stations were the following distances from the reactor bottom: Z, 0 m; RB3, 0.1 m; RB4, 0.35 m; RB5, 0.8 m; RB7, 2.5 m; RB9, 5.5 m; and RA4, 0.35 m.
b
ND, not determined.
c
The RT-PCR products were cloned and screened for variety by RFLP analysis, and selected clones were sequenced.