Table 5.

Measured and predicted thermodynamic parameters for test sequence duplex formation^a

	Average of curve fits				${\text{T}}_{\text{M}}^{-1}$ versus log Ct plots
Sequences	−ΔH° (kcal/mol)	−ΔS° (eu)	$-\Delta {\text{G}}_{37}^{\text{o}}$ (kcal/mol)	TMb (°C)	−ΔH° (kcal/mol)	−ΔS° (eu)	$-\Delta {\text{G}}_{37}^{\text{o}}$ (kcal/mol)	TMb (°C)
CGAGCGG	56.1	152.1	9.0	51.0	56.6	153.6	8.9	50.8
UUGCUCG	(57.0)	(156.6)	(8.4)	(47.7)
	(59.5)	(163.3)	(8.9)	(49.7)
CGAGCGG	53.6	145.8	8.4	48.4	48.9	130.9	8.3	48.7
GUGCUCG	(57.0)	(156.6)	(8.4)	(47.7)
	(59.5)	(163.3)	(8.9)	(49.7)
GCUCGGA	59.5	162.2	9.2	51.4	60.9	166.5	9.2	51.5
CGCGAGC	(59.8)	(163.5)	(9.1)	(50.9)
	(62.3)	(170.2)	(9.6)	(52.7)
CUGUGAA	61.0	173.2	7.3	40.8	56.0	157.1	7.2	40.9
CAGACAC	(54.0)	(151.9)	(6.6)	(38.7)
	(56.4)	(158.6)	(7.2)	(41.2)
UGCUAAC	71.7	208.0	7.2	39.9	64.0	183.0	7.2	40.5
UGACGAU	(42.8)	(120.1)	(5.6)	(30.2)
	(47.8)	(133.5)	(6.6)	(36.3)

Values in parenthesis are predicted: top row is the predicted values for single 3′ terminal overhang duplexes and bottom row is the predicted values as described in the text for duplexes with 3′ double overhangs.

^aSolutions are 1.0 M NaCl, 10 mM sodium cacodylate, 0.5 mM EDTA pH 7.

^bCalculated at 10⁻⁴ M oligomer concentration.