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Vinblastine binding is increased in cells overexpressing stathmin. BT20V1 (V1), BT20V3 (V3), BT20ST1 (ST1), and BT20ST3 (ST3) cells plated in tissue culture glass slide chambers were allowed to attach for 24 h and then treated for one hour with 20 μM fluorescein-conjugated vinblastine. Live cells were viewed with a fluorescent microscope under 40× magnification.
