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Gel electrophoresis of PCR products from serial dilutions of DNA extracts from different bacteria using the AF 1 plus AF 2 primer pair. (a) P. larvae subsp. larvae; (b) P. larvae subsp. pulvifaciens; (c) P. alvei; (d) B. cereus; (e) B. megaterium; (f) B. subtilis. Lanes (from left to right): molecular size marker (100-bp ladder), undiluted DNA, 10-fold dilution steps 10−1 to 10−10, negative control.
