TABLE 4.

Comparison of the efficacy of PCR, isolation, and BioLog identification techniques for the detection of P. larvae in bee products

Sample	Code	Type of sample	Country of origin	PCR results (AF 6 + AF 7)	Isolation (CFU/plate)	BioLog probability (%)
1	2001/36	Honey	Austria	+	>50	100
2	2001/37	Honey	Austria	−	3.55	98
3	2001/89	Honey	Austria	+	>50	100
4	2001/87	Honey	Austria	+	1.25	91
5	2001/983	Honey	Austria	−	33.8	100
6	2000/1019	Honey	Austria	+	0.8	100
7	2000/1020	Honey	Austria	+	1	91
8	B/768/92	Honey	Austria	+	1	100
9	AZ2000/696	Honey	Hungary	+	91	100
10	AZ2000/695	Honey	Austria	−	3.1	100
11	AZ2000/692	Honey	United Kingdom	−	5	100
12	AZ2000/691	Honey	France	+	>50	100
13	AZ2000/497	Honey	Saudi Arabia	+	10	99
14	AZ2000/1112	Honey	Turkey	+	>50	100
15	AZ98/76	Honey	USA	+	3.5	100
16	AZ95/5	Honey	Canada	+	3.35	100
17	AZ95/22	Honey	Romania	+	39	95
18	AZ95/4	Honey	Mexico	−	17	75
19	BP10/2001	Honey	Sardinia/Italy	+	1.58	100
20	AZ2001/94	Honey	Austria	+	6.2	99
21	BP28/2001	Honey	Austria	+	2	96
22	BP31/2001	Honey	Austria	+	>50	99
23	ÜW3	Honey	Austria	+	3.6	96
24	BP6/2000	Honey	Austria	−	0	0
25	AZ2001/280	Honey	India	−	0	0
26	AZ2001/325	Honey	India	−	0	0
27	ÜW5	Pollen	Germany	+	4.5	100
28	BP79/2000	Pollen	The Netherlands	+	0.25	100
29	W8	Wax	Austria	+	0.2	91
30	Wabe1	Brood	Austria	+	>50	100