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. 2003 Mar;69(3):1435–1441. doi: 10.1128/AEM.69.3.1435-1441.2003

TABLE 2.

Primer sequences and amplification cycling conditions for the different PCR-based genomic DNA fingerprints

Primer set Primer Nucleotide sequence References Amplification cycling conditions
ERIC ERIC-1R ERIC-2 5′-ATG TAA GCT CCT GGG GAT TCA C-3′ 5′-AAG TAA GTG ACT GGG GTG AGC G-3′ 1, 19, 30 Initial denaturation 95°C for 2 min, 92°C for 30 s; 35 cycles of 92°C for 30 s, 50°C for 80 s, and 68°C for 200 s, final extension at 68°C for 8 min, final 4°C soak
REP REP 1R DT REP 2-DT 5′-III ICG ICG ICA TCI GGC-3′ 5′-ICG ICT TAT CIG GGC TAC-3′ 1, 30 Initial denaturation at 95°C for 2 min, 92°C for 30 s, 35 cycles of 92°C for 30 s, 38°C for 80 s, and 68°C for 200 s, final extension at 68°C for 8 min, final 4°C soak
BOXAIR 5′-CTA CGG CAA GGC GAC GCT GAC G-3′ 2, 24 Initial denaturation 95°C for 2 min, 94°C for 3 s; 30 cycles of 92°C for 30 s, 50°C for 1 min, and 65°C for 8 min; final extension at 68°C for 8 min, final 4°C soak