TABLE 2.
Primer sequences and amplification cycling conditions for the different PCR-based genomic DNA fingerprints
Primer set | Primer | Nucleotide sequence | References | Amplification cycling conditions |
---|---|---|---|---|
ERIC | ERIC-1R ERIC-2 | 5′-ATG TAA GCT CCT GGG GAT TCA C-3′ 5′-AAG TAA GTG ACT GGG GTG AGC G-3′ | 1, 19, 30 | Initial denaturation 95°C for 2 min, 92°C for 30 s; 35 cycles of 92°C for 30 s, 50°C for 80 s, and 68°C for 200 s, final extension at 68°C for 8 min, final 4°C soak |
REP | REP 1R DT REP 2-DT | 5′-III ICG ICG ICA TCI GGC-3′ 5′-ICG ICT TAT CIG GGC TAC-3′ | 1, 30 | Initial denaturation at 95°C for 2 min, 92°C for 30 s, 35 cycles of 92°C for 30 s, 38°C for 80 s, and 68°C for 200 s, final extension at 68°C for 8 min, final 4°C soak |
BOXAIR | 5′-CTA CGG CAA GGC GAC GCT GAC G-3′ | 2, 24 | Initial denaturation 95°C for 2 min, 94°C for 3 s; 30 cycles of 92°C for 30 s, 50°C for 1 min, and 65°C for 8 min; final extension at 68°C for 8 min, final 4°C soak |