TABLE 1.

Primers and probes used for PCR, FISH, and real-time PCR

Name	Use	E. coli 16S rRNA gene positions	Reference or sequence (5′ to 3′), if designed for this study
Bac-8F	Bacterial real-time PCR primer	8-27	3
Bac-515R	Bacterial real-time PCR primer	515-533	12
Bac-338R	Bacterial and I025 real-time PCR probe and FISH probe	338-355	17
TM7-910F	TM7 real-time PCR primer	930-910	CAT AAA GGA ATT GAC GGG GAC
TM7-1177R	TM7 PCR and real-time PCR primer	1177-1200	GAC CTG ACA TCA TCC CCT CCT TCC
TM7-1093F	TM7 real-time PCR probe	1093-1113	AGT CCA TCA ACG AGC GCA ACC
TM7-905	TM7 FISH probe	905-926	9
I025-135F	I025 real-time PCR primer	135-154	CCC TGC AGT GAG GGA TAA GA
I025-590R	I025 real-time PCR primer	590-611	GTT TTC ATC GCT CGC TAA CTT G
I025-136	I025 FISH probe	136-155	GTC TTA TCC CTC ACT GCA GG
Control probe	Negative control for FISH	519-536	20