Figure 1.

dTAK1 mutations block the expression of antibacterial peptide genes. (A) Diagram of genetic lesions in dTAK1 alleles used in this study. Roman numerals represent the kinase subdomains. (B) Northern blot comparison of dTAK1¹⁷⁹ with mutations in Imd pathway genes. (C) Northern blot analysis of dTAK1 mutants and complementation test. Adult flies of the indicated genotypes were infected with E. coli and then incubated for 12 h (B) or overnight (C) at 25°C. RNA was then prepared and analyzed as described in Materials and methods.