Table 3.

Kinetic parameters of COPII turnover simulation under control and sterol depleted conditions

Parametera	Control	LDS/HPCD	Ratio [LDS/HPCD:control]
R	0.408±0.176	0.384±0.144	0.941
k	0.162±0.071	0.163±0.076	1.006
Γ	1.706±1.019	1.333±0.608	0.781
ω	0.406±0.270	0.242±0.099	0.596
γ	0.386±0.088	0.339±0.081	0.878
λ	0.170±0.029	0.113±0.018	0.665
S*/(S*+Cm)	0.468±0.100	0.476±0.084	1.017
aParameter space that reproduced the experimental data. The range still consistent with these constraints is given by the ‘±’ values. Parameters were defined as: R, ratio of total cellular Sec23/24p to Sar1p; k, Sar1p-GDP binding to ERES; Γ, release of Sar1p-GDP from ERES; ω, GTP-exchange rate; γ, rate of GTP-hydrolysis on Sar1p; λ, release of Sec23/24p from ERES; S*/(S*+Cm), ratio of membrane bound Sar1p-GDP relative to total Sar1p at ERES (for details, see Forster et al, 2006).