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. 2006 Jun 8;25(13):3191–3202. doi: 10.1038/sj.emboj.7601179

Figure 4.

Figure 4

Occupancy of the chn promoter by Ebi, SMRTER and NICD in vivo. (A) ChIP assay of third instar eye imaginal discs using anti-Ebi, anti-SMRTER or anti-NICD. PR(chn) and Int(chn) are derived from the chn genomic region (Figures 1A and 3A), and hsp27 is a fragment of the hsp27 promoter region (Tsai et al, 1999; Sawatsubashi et al, 2004). E(spl)mδ is a fragment of the E(spl)mδ promoter region (Cooper and Bray, 1999). (B) Sequential ChIP assay. Crosslinked chromatin was immunoprecipitated with anti-Ebi followed by a second immunoprecipitation with anti-SMRTER or anti-NICD. MS is the sequence from microsatellite DNA (Sawatsubashi et al, 2004). Although anti-SMRTER precipitated PR(chn) from Ebi-containing chromatin, anti-NICD did not.