Figure 4.
Complementation of mitochondrial morphology by L-OPA1 in OPA1-RNAi cells. (A) HeLa cells were transfected with the siRNA for GFP (cont.) or for human OPA1 three times with 48 h intervals. Then the indicated constructs were transfected and cultured for 18 h. The cells were stained with MitoTracker. (B) HeLa cells with filamentous-network, intermediate, or completely fragmented mitochondrial structures in (A) were counted. At least 100 cells were counted from three different optical fields.