Figure 5.

Association of the Fluo-4 phenotype with pfmdr1 polymorphisms. (A) Fluo-4 staining images and ratios of the mean fluorescence signals in the vacuolar and cytoplasmic regions (Fluo-4 R_vac/cyt), observed in different pfmdr1 allelic exchange mutants generated on either a GC03, 3BA6, 7G8 or a D10 genetic background (Reed et al, 2000; Sidhu et al, 2005; Supplementary Table II). NS, not significant as compared to GC03, 3BA6, 7G8 or D10. Means±s.e. of over 12 independent determinations collected over several days are shown. (B) Fluo-4 staining images and Fluo-4 R_vac/cyt ratios observed in different pfcrt allelic exchange mutants (Sidhu et al, 2002). C4^Dd2 and C6^7G8 contain the Southeast Asian/African and Latin American pfcrt alleles from Dd2 and 7G8, respectively (Sidhu et al, 2002). GC03 is the parental clone and C2^GC03 is a control retaining the pfcrt allele of GC03. NS, not significant (compared to GC03). The means±s.e. of over 14 independent determinations collected over several days are shown. ^**P<0.001; NS, not significant. Bar, 5 μm.