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. 2000 Oct;11(10):3509–3523. doi: 10.1091/mbc.11.10.3509

Figure 7.

Figure 7

The overexpression of Bcl-x(L) partially inhibits the apoptotic effects of β-catenin. (A) Full-length (FL) and Arm β-catenin induce apoptosis in HeLa cells and HeLa cells overexpressing Bcl-x(L). Only a few cells (arrows) contain the exogenous GFP marker after 7 d. Bar, 50 μm. (B) These apoptotic effects are retarded in HeLa cells overexpressing Bcl-x(L). After 7 d of transfection, parental HeLa cells showed very few BFP-positive cells (overexpressing β-catenin), whereas 10–15% of HeLa cells overexpressing Bcl-x(L) (HeLa-Bcl) still showed BFP-positive cells expressing either full-length (FL) or Arm β-catenin. (C) Western blot analyses showed that full-length β-catenin–GFP can still be detected in HeLa cells overexpressing Bcl-x(L) 8 d after transfection, albeit in lesser amounts than on d 2. Because of the lack of a binding epitope of Arm β-catenin and a similar molecular weight with endogenous β-catenin, it was difficult to distinguish Arm β-catenin from endogenous β-catenin by anti-β-catenin antibody (top panel). However, blotting with anti-GFP antibody clearly showed the Arm β-catenin band at d 2 and a decreased band at d 8 in HeLa cells overexpressing Bcl-x(L). Neither band was detectable in HeLa parental cells at d 8. Con, control HeLa cell extracts without transfection; HeLa, HeLa parental cells; Bcl-x(L), HeLa cells overexpressing Bcl-x(L); FL, full-length β-catenin transfection; Ar., Arm β-catenin transfection; Endo., endogenous β -catenin.